CALL FOR PAPERS Control Mechanisms of Renin Synthesis and Release: A 21st Century Perspective NO and cGMP mediate angiotensin AT2 receptor-induced renal renin inhibition in young rats

Siragy HM, Inagami T, Carey RM. NO and cGMP mediate angiotensin AT2 receptor-induced renal renin inhibition in young rats. Am J Physiol Regul Integr Comp Physiol 293: R1461–R1467, 2007. First published August 1, 2007; doi:10.1152/ajpregu.00014.2007.—We hypothesized that angiotensin subtype-2 receptor (AT2R) inhibits renal renin biosynthesis in young rats via nitric oxide (NO). We monitored changes in renal NO, cGMP, renal renin content (RRC), and ANG II in 4-wk-old rats in response to low sodium (LNa ) intake alone and combined with 8-h direct renal cortical administration of AT1 receptor blocker valsartan (VAL), AT2R blocker PD123319 (PD), NO synthase inhibitor N-nitro-L-arginine methyl ester (L-NAME), NO donor S-nitroso-N-acetyl penicillamine (SNAP), or guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo[4,2] quinoxaline-1-one (ODQ). In addition, we monitored renal endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) in response to VAL or PD. LNa , VAL, PD, L-NAME, and ODQ increased RRC, ANG II, and renin mRNA. PD and L-NAME decreased NO and cGMP, while SNAP reduced RRC, ANG II, renin mRNA, and reversed the effects of PD. PD also reduced eNOS and nNOS protein and mRNA. Combined treatment with PD, L-NAME, or ODQ and VAL reversed the effects of VAL and caused further increase in RRC, ANG II, renin mRNA, and protein. ODQ reversed the effects of SNAP. These data demonstrate that the renal AT2 receptor decreases renal renin biosynthesis and ANG II production in young rats. Reversal of the PD effects by SNAP and SNAP effects by ODQ confirms that NO and cGMP mediate the AT2 receptor inhibition of renal renin production.